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Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells

dc.contributor.authorBustillo, Soledad
dc.contributor.authorGay, Claudia C.
dc.contributor.authorGarcía Denegri, María Emilia
dc.contributor.authorPonce-Soto, Luis Alberto
dc.contributor.authorBal de Kier Joffé, Elisa Dora
dc.contributor.authorAcosta, Ofelia Cristina
dc.contributor.authorLeiva, Laura Cristina Ana
dc.date.accessioned2025-12-15T11:31:17Z
dc.date.available2025-12-15T11:31:17Z
dc.date.issued2012-02
dc.identifier.citationBustillo, Soledad, et al., 2012. Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells. Toxicon. Oxford: Elsevier, vol. 59, p. 338–343. ISSN 1879-3150es
dc.identifier.issn0041-0101es
dc.identifier.urihttp://repositorio.unne.edu.ar/handle/123456789/59352
dc.description.abstractAcute muscle damage, myonecrosis, is one of the main characteristics of envenoming by Bothrops genus. In this in vitro study we investigated the role of a metalloproteinase (baltergin) and an acidic phospholipase A2 (Ba SPII RP4) in the cytotoxicity exhibited by Bothrops alternatus venom. Baltergin metalloproteinase purified from the venom exerted a toxic effect on C2C12 myoblast cells (CC50: 583.34 mg/mL) which involved morphological alterations compatible with apoptosis/anoikis. On the contrary, the most abundant PLA2 isolated from this venom did not exhibit cytotoxicity at times and doses tested. However, when myoblasts were treated with both enzymes together, synergic activity was demonstrated. Neutralization of the venom with specific antibodies (IgG anti-baltergin and IgG anti-PLA2) confirmed this synergism.es
dc.formatapplication/pdfes
dc.format.extentp. 338-343es
dc.language.isoenges
dc.publisherElsevieres
dc.relation.urihttps://doi.org/10.1016/j.toxicon.2011.11.007es
dc.rightsopenAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/es
dc.sourceToxicon, 2012, vol. 59, p. 338–343.es
dc.subjectBothrops alternatuses
dc.subjectCytotoxicityes
dc.subjectPhospholipase A2es
dc.subjectMetalloproteinasees
dc.subjectBaltergines
dc.subjectC2C12es
dc.subjectSynergismes
dc.titleSynergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cellses
dc.typeArtículoes
unne.affiliationFil: Bustillo, Soledad. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.es
unne.affiliationFil: Gay, Claudia Carolina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.es
unne.affiliationFil: García Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas, Naturales y Agrimensura; Argentina.es
unne.affiliationFil: García Denegri, María Emilia. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.es
unne.affiliationFil: Ponce-Soto, Luis Alberto. Universidade Estadual de Campinas. Instituto de Biología; Brasil.es
unne.affiliationFil: Bal de Kier Joffé, Elisa Dora. Instituto de Oncología Angel H. Roffo; Argentina.Fil: Acosta, Ofelia Cristina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.es
unne.affiliationFil: Leiva, Laura Cristina Ana. Instituto de Oncología Angel H. Roffo; Argentina.es
unne.journal.paisReino Unidoes
unne.journal.ciudadOxfordes
unne.journal.volume59es
unne.journal.number2es
unne.ISSN-e1879-3150es


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