Gametogenesis and reproductive dynamics of Scinax acuminatus (Anura: Hylidae) : morphological, histological and immunohistochemical analysis
Fecha
2021Autor
Olea, Gabriela Beatriz
Cheij, Esteban Omar
Cuzziol Boccioni, Ana Paula
Rodríguez, Florencia Evelyn
Céspedez, Jorge Abel
Lombardo, Daniel Marcelo
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To characterize the reproductive biology of Scinax acuminatus and contribute
to the natural history of this species, the morphology of the reproductive system of
males and females was analyzed at anatomical, histological and immunohistochemical
levels. The individuals were collected fortnightly between August and December
(2016) and January to December (2018). The anatomy of the reproductive system was
analyzed in a stereoscopic microscope, and histological preparations staining with
hematoxylin-eosin and Masson’s trichromic, PAS and Coomassie Blue was performed
as well. To characterize the meiotic-active cells in the testes, immunostaining with the
PCNA proliferation protein was performed. There were found females with ovaries with
oocytes in different stages of maturity and post-ovulatory females. Males presented
continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA
in spermatogonia during the cycle. The results of this work serve as a basis for the
characterization of the reproductive cycle in S. acuminatus and provide background
information on the analysis of spermatogenic activity by IHQ from the study of the
immunodetection of the PCNA cell proliferation protein. Future studies will focus on the
evaluation of cell death processes during the reproductive cycle in the studied species
to compare with those obtained in terms of cell proliferation.
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