Plant regeneration of ilex paraguariensis (aquifoliaceaé) by in vitro culture of nodal segments
Fecha
2020-04Autor
Sansberro, Pedro Alfonso
Rey, Hebe Yolanda
Bernardis, Aldo Ceferino
Luna, Claudia Verónica
Collavino, Mónica Mariana
Mroginski, Luis Amado
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In vitro regeneration of complete plants ffom nodal single bud segments of “yerba mate” {Ilex
paraguariensis St. Hil.) was studied under defined nutritional and environmental conditions. Nodal segments harvested ffom actively growing shoots o f conventionally raised plants were cultured on nutrient médium with the mineral salts and vitamins o f Murashige and Skoog médium at 1/4 strength, supplemented with various concentrations o f sucrose and 6-benzyladenine (BAP). Shoot regeneration ffom explants o f both young (2 years oíd) and adult (20 years oíd) mother plants were readily achieved in the médium supplemented with 0.04 - 0.09 M sucrose with or without BAP. As many as 60 - 65% o f the nodal segments cultured formed shoots. Rooting o f regenerated shoots was observed in 50% o f the explants harvested ffom young plants, whereas 25% o f the explants rooted when the nodal explants were harvested ffom adult plants. The best rooting induction was achieved on 1/4 strength MS médium with vermiculite as the substrate and supplemented with 1 - 1.5% IBA (indolebutyric acid) and 1 -
2% PPZ (3- methyl - 1 - phenyl - 2 pyrazolin - 5 - one). Plantlets were successfully transferred to soil.
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