Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
Espasandin, Fabiana D.
Calzadilla, Pablo I.
Maiale, Santiago J.
Ruiz, Oscar A.
Sansberro, Pedro A.
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To analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild-type and transgenic lines harbouring the pRD29A: oat arginine decarboxylase (ADC, EC 18.104.22.168) construct were subjected to a gradual increase in salinity that was applied gradually by means of sodium chloride irrigation (from 0 to 0.3 mol L−1) every 5 days up to the maximum concentration. At the end of the experiment, the transgenic lines were healthier than the wild-type plants and displayed a smaller reduction in shoot biomass and a slight increase in root growth in response to stress. The overexpression of ADC increased osmotic adjustment (5.8- fold) via the release of proline. The salinity treatment doubled the potassium uptake by roots from transgenic ADC stressed plants with a concomitant decrease in the accumulation of sodium, balancing the Na+/ K+ ratio. Analysis of gene expression, enzymatic activities and hormone metabolism suggests a crosstalk between polyamines and abscisic acid in response to salinity via modulation of the abscisic acid biosynthesis-related enzyme 9-cis-epoxycarotenoid dioxygenase (EC 22.214.171.124) at the transcriptional level.
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