Localization and distribution of CCK-8-, NPY-, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836)
Fecha
2020-12Autor
Barrios, Carlos Eduardo
Santinón, Juan José
Domitrovic, Hugo Alberto
Sánchez, Sebastián
Henández, David Roque
Metadatos
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This study describes the histological characteristics and distribution of
gastrointestinal tract endocrine cells (ECs) of Prochilodus lineatus (detritivorous fish)
using immunohistochemical procedures. The digestive tract of P. lineatus was divided
into seven portions: stomach (cardial and pyloric), pyloric caeca, and intestine (anterior,
glandular, middle and posterior). A pool of specific antisera against cholecystokinin
(CCK-8), -neuropeptide Y (NPY), -ghrelin (Ghre) and -leu-enkephalin (Leu-ENK) to
identify ECs were used. According to the morphological characteristics of ECs, two
different types were identified and classified as open or closed-type. The number of
ECs varied throughout the gastrointestinal tract, though a high abundance was found
in the anterior intestine and pyloric caeca. A large number of ECs immunoreactive
to CCK-8 and NPY were recorded in the anterior, glandular and middle intestine. ECs
immunopositive to Leu-ENK were distributed in the stomach and pyloric caeca. For Ghre,
immunopositive ECs were restricted to the glandular intestine. The results of the present
study indicate that P. lineatus presents an ECs distribution pattern with species-specific
particularities. However, CCK showed a distribution similar to that of omnivores, which is
possibly related to local signaling functions in order to achieve the correct digestion of
the various organisms found in the detritus.
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