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dc.contributor.authorTodaro, Juan Santiago
dc.contributor.authorStoyanoff, Tania Romina
dc.contributor.authorAguirre, María V.
dc.contributor.authorBrandan, Nora C.
dc.date.accessioned2022-04-19T14:42:54Z
dc.date.available2022-04-19T14:42:54Z
dc.date.issued2013
dc.identifier.citationTodaro, Juan Santiago, et al, 2013. Molecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleeding. Journal of Biological Sciences. New York: Science Alert, vol. 13, no. 6, p. 452-462. ISSN 1812-5719.es
dc.identifier.issn1727-3048es
dc.identifier.urihttp://repositorio.unne.edu.ar/handle/123456789/33893
dc.description.abstractThe underlying interactions among apoptosis, eiythroid proliferation and differentiation involved in bone marrow eiythropoietic response after an acute blood-loss have not yet been elucidated in detail. We hypothesized that Erythropoietin receptor, Bax, caspase-3, cytochrome c, Smac/DIABLO and Bcl-xL molecules play important roles at time of acute eiythropoietic need to ameliorate the hypoxic stress. Experiments were performed using in vivo murine model of anaemia induced by blood-loss in a time course study of 15 days. Haematological parameters and bone marrow cellularities were determined. Bone marrow apoptotic assays included: double fluorescent staining (acridine orange/ethidium bromide) and TUNEL. Bone marrow clonogenic assays were performed for evaluating eiythroid colony forming unif s expansión. The Eiythropoietin receptor, Bax, Bcl-xu Smac/DIABLO, caspase-3 and cytochrome c expressions were assessed by immunoblottings. Caspase-3 activity was determined with a colorimetric assay kit. Bleeding induces bone marrow apoptosis from 1 to 3 days, concomitant with Bax over-expression and Bcl-xL decrease. The mitochondrial dysfunction caused cytochrome c and Smac/DIABLO release to cytosol and caspase-3 activation. Eiythropoietic recoveiy was associated with Eiythropoietin receptor over expression from the third day, concomitant with the eiythroid progenitors and Bcl-xL/Bax ratio enhancements. Eiythropoiesis after bleeding depends on a delicate balance among proapoptotic (Bax, caspase-3, cytochrome c, Smac/DIABLO) and prosurvival proteins (Eiythropoietin receptor, Bcl-xL), as the crucial regulators in bone marrow eiythroid recoveiy. These findings provide new insights into the homeostatic mechanisms which promote eiythropoietic response post-bleeding.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherScience Alertes
dc.rightsopenAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/es
dc.sourceJournal of Biological Sciences, 2013, vol. 13, no. 6, p. 452-462.es
dc.subjectApoptosises
dc.subjectBleedinges
dc.subjectBone marrowes
dc.subjectBaspase-3-erythropoietin receptores
dc.titleMolecular mechanisms involved in murine bone marrow erythropoietic response to acute anaemia by bleedinges
dc.typeArtículoes
unne.affiliationFil: Todaro, Juan Santiago. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.es
unne.affiliationFil: Stoyanoff, Tania Romina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.es
unne.affiliationFil: Aguirre, María V. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.es
unne.affiliationFil: Brandan, Nora Cristina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.es
unne.journal.paisEstados Unidoses
unne.journal.ciudadNew Yorkes
unne.ISSN-e1812-5719es


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