In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions (with 1 table & 2 figures)
Fecha
2004Autor
Scocchi, Adriana M.
Mroginski, Luis Amado
Metadatos
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Studies on in vitro storage of Melia azedarach –“paradise tree”- under
slow-growth conditions were carried out to develop an efficient protocol for the con-
servation of the genetic diversity of the crop. The response to different growth re-
tardation treatments was examined using clone E. In vitro cultures of apical mer-
istem-tips (0.2-0.3 mm in length) could be effectively maintained for 12 months at
4oC on 1⁄4MS basal medium supplemented with 0.5μM BA. Shoot proliferation was
observed on 67% of the recovered apical meristem-tips, after 12 months at 4oC.
Rooting of the regenerated shoots was achieved by reculturing them on MS + 17.5
μM IBA, during 4 days and subsequently transferred to MS medium lacking growth-
regulators for 30 days. Complete plants were successfully transferred to the green-
house.
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