Mostrar el registro sencillo del ítem
Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
dc.contributor.author | Acevedo, Raúl Maximiliano | es |
dc.contributor.author | Avico, Edgardo Hernán | es |
dc.contributor.author | Ruiz, Oscar Adolfo | es |
dc.contributor.author | Sansberro, Pedro Alfonso | es |
dc.date.accessioned | 2020-06-02T22:48:48Z | |
dc.date.available | 2020-06-02T22:48:48Z | |
dc.date.issued | 2018 | es |
dc.identifier.citation | Acevedo, Raúl Maximiliano, et al, 2018. Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought. Biologia Plantarum. República Checa: Czech Academy of Sciences. Institute of Experimental Botany, vol. 62, no. 1, p. 89-96. ISSN 1573-8264. | |
dc.identifier.issn | 0006-3134 | |
dc.identifier.uri | http://repositorio.unne.edu.ar/handle/123456789/9149 | |
dc.description.abstract | Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, it requires a normalization using internal reference genes with stable expression. To address this issue, nine candidate reference genes were investigated in Ilex paraguariensis leaves subjected to water stress. To facilitate the selection, we analysed the real-time qPCR data with three different software programs. The obtained results support the conclusion that RNA polymerase associated protein rtf1 homolog (RTF) combined with any of the following pairs is the most suitable triad of genes to compute a normalization factor: elongation factor 1-alpha + tubulin alpha chain (EF1a + α-Tub), actin + cyclophilin 38 (ACT + CYP38), or cyclophilin 38 + vacuolar protein sorting-associated protein 18 homologs (CYP38 + VPS). Our analysis constitutes the first in-depth study to identify the appropriate reference genes for the quantification of transcription in Ilex paraguariensis leaves during drought and provides essential information for further gene expression studies in this tree species | es |
dc.format | application/pdf | |
dc.format.extent | p. 89-96 | |
dc.language.iso | eng | es |
dc.publisher | Springer | es |
dc.relation | https://bp.ueb.cas.cz/pdfs/bpl/2018/01/09.pdf | |
dc.rights | openAccess | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
dc.source | Biologia Plantarum, 2018, vol. 62, no. 1, p. 89-96. | |
dc.subject | Abiotic stress | es |
dc.subject | Gene expression | es |
dc.subject | House-keeping genes | es |
dc.title | Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought | es |
dc.type | Artículo | es |
dc.description.affiliation | Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biotecnológicas; Argentina. | es |
dc.description.affiliation | Fil: Acevedo, Raúl Maximiliano. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. | |
dc.description.affiliation | Fil: Acevedo, Raúl Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. | |
dc.description.affiliation | Fil: Avico, Edgardo Hernán. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. | |
dc.description.affiliation | Fil: Avico, Edgardo Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. | |
dc.description.affiliation | Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas; Argentina. | |
dc.description.affiliation | Fil: Ruiz, Oscar Adolfo. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín"; Argentina. | |
dc.description.affiliation | Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. | |
dc.description.affiliation | Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. | |
unne.journal.pais | República Checa | |
unne.journal.ciudad | Rozvojová | |
unne.ISSN-e | 1573-8264 |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
Artículos de revista [476]