Plant regeneration from ilex spp. (aquifoliaceae) in vitro

Abstract

In vitro plant regeneration from nodal segments (containing one axillary bud) of seven species of the genus Ilex (l argentina, I. brevicuspis, I. dumosa, I. microdonta, I. pseudoboxus, I. taubertianb and I theezans ) were readily achieved through three steps: 1) shoot regeneration by in vitro culture of nodal segments in MS médium at 1/4 strength, plus 3% sucrose and 0.65% agar (1/4MS) and 0.5 |jM BA (45 days of culture); 2) Induction of rooting from regenerated shoots with 1/4MS (solidified with 2.5 g.L'1 “Phytagel”) with 7.3 pM IBA (7 days) and, 3) subculture of shoot on a fresh médium (1/4MS lacking plant growth regulators) during 21 days. Shoot regeneration of other three species ( I aquifolium, /. bmsiliensis and /. inlegerrima) were also obtained by in vitro culture of nodal segments. Shoot regeneration of/. aquifolium, I. brasiliensis, I. integerrima, I. microdonta, I. pseudoboxus, and I. taubertiana were also obtained by culture shoot tips on 1/4MS and 0.5 ]iM BA. Shoot regeneration from meristems o f l argentina, I. brevicuspis, I. dumosa, and I. theezans were readily achieved by in vitro culture on the same médium.