Characterization and expression analysis of somatic embryogenesis receptor kinase (SERK) genes in sexual and apomictic paspalum notatum
Fecha
2014Autor
Podio, Maricel
Felitti, Silvina Andrea
Siena, Lorena Adelina
Delgado Benarroch, Luciana
Mancini, Micaela
Seijo, José Guillermo
González, Ana María
Pessino, Silvina Claudia
Ortíz, Juan Pablo Amelio
Metadatos
Mostrar el registro completo del ítemResumen
The SOMATIC EMBRYOGENESIS RECEPTOR-
LIKE KINASE (SERK) gene plays a fundamental role in
somatic embryogenesis of angiosperms, and is associated
with apomixis in Poa pratensis. The objective of this work
was to isolate, characterize and analyze the expression pat-
terns of SERK genes in apomictic and sexual genotypes of
Paspalum notatum. A conserved 200-bp gene fragment was
amplified from genomic DNA with heterologous primers,
and used to initiate a chromosomal walking strategy for
cloning the complete sequence. This procedure allowed the
isolation of two members of the P. notatum SERK family;
PnSERK1, which is similar to PpSERK1, and PnSERK2,
which is similar to ZmSERK2 and AtSERK1. Phylogenetic
analyses indicated that PnSERK1 and PnSERK2 represent
paralogous sequences. Southern-blot hybridization indi-
cated the presence of at least three copies of SERK genes
in the species. qRT-PCR analyses revealed that PnSERK2
was expressed at significantly higher levels than PnSERK1
in roots, leaves, reproductive tissues and embryogenic calli.Moreover, in situ hybridization experiments revealed that
PnSERK2 displayed a spatially and chronologically altered
expression pattern in reproductive organs of the apom-
ictic genotype with respect to the sexual one. PnSERK2
is expressed in nucellar cells of the apomictic genotype
at meiosis, but only in the megaspore mother cell in the
sexual genotype. Therefore, apomixis onset in P. notatum
seems to be correlated with the expression of PnSERK2 in
nucellar tissue.
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