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Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
(Springer Nature, 2019-05)
Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence
of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, ...
Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
(Springer, 2018)
Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, ...
Gene expression changes in response to drought stress in Ilex paraguariensis leaves
(Southerm Cross, 2016-10)
Ilex paraguariensis St. Hil. is recognized as a shade-tolerant tree, cultivated in north-eastern Argentina, south-eastern Brazil, and Paraguay. Its leaves and young shoots are industrialised and; hence, being used to prepare ...
Isolation of high quality RNA from soil-grown Ilex paraguariensis roots suitable for next-generation sequencing and gene expression analyses
(Southerm Cross, 2017-07)
Extraction of high quality RNA is a prerequisite for downstream application in functional genomics analyses. However, the extraction and purification of pure nucleic acids from root tissues is generally difficult due to ...