Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
Fecha
2019-05Autor
Acevedo, Raúl Maximiliano
Avico, Edgardo Hernán
González, Sergio
Acácio Rodrígues, Salvador
Rivarola, Máximo
Paniego, Norma
Nunes Nesi, Adriano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
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Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence
of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated
(1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation
of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed
transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts
involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water
loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional
regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously,
significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels
were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic
profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds
to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms
associated with drought response in perennial subtropical species.
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