Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
Fecha
2017-05-12Autor
Espasandin, Fabiana Daniela
Calzadilla, Pablo I.
Maiale, Santiago Javier
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
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To analyse the putative effects of putrescine on
the plant response to salinity, micropropagated Lotus tenuis
plants from wild-type and transgenic lines harbouring the
pRD29A: oat arginine decarboxylase (ADC, EC 4.1.1.19)
construct were subjected to a gradual increase in salinity
that was applied gradually by means of sodium chloride
irrigation (from 0 to 0.3 mol L−1) every 5 days up to the
maximum concentration. At the end of the experiment, the
transgenic lines were healthier than the wild-type plants
and displayed a smaller reduction in shoot biomass and
a slight increase in root growth in response to stress. The
overexpression of ADC increased osmotic adjustment (5.8-
fold) via the release of proline. The salinity treatment doubled
the potassium uptake by roots from transgenic ADC
stressed plants with a concomitant decrease in the accumulation
of sodium, balancing the Na+/
K+ ratio. Analysis of
gene expression, enzymatic activities and hormone metabolism
suggests a crosstalk between polyamines and abscisic
acid in response to salinity via modulation of the abscisic
acid biosynthesis-related enzyme 9-cis-epoxycarotenoid
dioxygenase (EC 1.13.11.51) at the transcriptional level.
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