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Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
dc.contributor.author | Cholich, Luciana Andrea | |
dc.contributor.author | Pistán, María Elena | |
dc.contributor.author | Torres, Ana María | |
dc.contributor.author | Ortega, Hugo Héctor | |
dc.contributor.author | Gardner, Dale R. | |
dc.contributor.author | Bustillo, Soledad | |
dc.date.accessioned | 2024-03-20T10:45:17Z | |
dc.date.available | 2024-03-20T10:45:17Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Cholich, Luciana Andrea., et. al., 2021. Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae). Revista de Biología Tropical. San José: Universidad de Costa Rica, vol. 69, no. 1, p. 197-2060. E-ISSN 2215-2075. | es |
dc.identifier.issn | 0034-7744 | es |
dc.identifier.uri | http://repositorio.unne.edu.ar/handle/123456789/53153 | |
dc.description.abstract | Introduction: Prosopis spp. pods have shown to be a potential source of protein and energy in livestock. However, prolonged ingestion of some of these species produces neurological symptoms in ruminants. Objective: In the present study, the alkaloid content and the in vitro neurotoxic activity of alkaloid enrichedextracts from P. flexuosa and P. nigra pods were determined in order to elucidate the mechanism of animal poisoning caused by these species. Methods: The main alkaloids present in both extracts were analysed by high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS). The cytotoxic activity of Prosopis alkaloid enriched-extracts in primary mixed glial cell culture was assessed by phase contrast microscopy and using neutral red, and lactate dehydrogenase (LDH) activity assays. Results: Juliprosine and juliprosopine were identified in P. flexuosa pods, while the absence of these alkaloids in P. nigra was confirmed. Both extracts (5-30 μg/mL) induced in a dose dependent manner, morphological alterations, such as swelling, enlargement and detachment from the culture surface. Consistent with this, decrease in cell viability and release of LDH 48 hours after exposure, revealed that P. flexuosa pods was significantly more cytotoxic than P. nigra. Conclusions: In P. flexuosa pods, juliprosine and juliprosopine alkaloids were identified for the first time. Moreover, the present study suggests that the cytotoxic effect displayed by both extracts is due to its alkaloid content. However, the presence of piperidine alkaloids in P. flexuosa could explain the greater cytotoxicity on glial cells with respect to P. nigra that was not shown to contain these alkaloids | es |
dc.format | application/pdf | es |
dc.format.extent | p. 197-206 | es |
dc.language.iso | eng | es |
dc.publisher | Universidad de Costa Rica | es |
dc.rights | openAccess | es |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | es |
dc.source | Revista de Biología Tropical, 2021, vol. 69, no. 1, p. 197-206. | es |
dc.subject | Algarrobos | es |
dc.subject | Cytotoxicity | es |
dc.subject | Piperidine alkaloids | es |
dc.subject | Prosopis species | es |
dc.title | Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae) | es |
dc.type | Artículo | es |
unne.affiliation | Fil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. | es |
unne.affiliation | Fil: Pistán, María Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. | es |
unne.affiliation | Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. | es |
unne.affiliation | Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. | es |
unne.affiliation | Fil: Gardner, Dale R. Agricultural Research Service U.S. Departament of Agriculture; Estados Unidos. | es |
unne.affiliation | Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. | es |
unne.journal.pais | Costa Rica | es |
unne.journal.ciudad | San José | es |
unne.journal.volume | 69 | es |
unne.journal.number | 1 | es |
unne.ISSN-e | 2215-2075 | es |
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