Storage of cohniella cepula (Orchidaceae) Pollinia : fertilizing ability and subsequent fruit and seed formation
Fecha
2016-10Autor
Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
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Pollen storage is of great importance for plant breeding and production besides
an efficient means for preservation of haploid gene pool of plant genetic resources and
rare or endangered species. Pollinia of Cohniella cepula were stored over 1 year at
4,L20,L70, andL196 8C. Fertilizing ability of fresh and stored (30 to 360 days) pollinia
was determined by the fruit and seed formation for each treatment, as well as by the
seed viability, in vitro seed germination, and seedling growth. Pollinia stored at L70
and L196 8C showed high fertilizing ability (94.4% to 100.0%) even 1 year after collection,
revealing no significant differences with fresh pollinia. Seeds from all treatments
showed high viability (91.2% to 94.3%) through the 2,3,5-triphenyltetrazolium chloride
(TTC) reduction assay and high in vitro germination (91.7% to 97.3%). Thus, successful
ultracold storage of C. cepula pollinia was feasible without any desiccation, cryoprotection,
or precooling treatment before placing into an ultra freezer (L70 8C) or immersing in
liquid nitrogen (LN) (L196 8C).
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