Listar por autor "Acevedo, Raúl Maximiliano"
Mostrando ítems 1-4 de 4
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Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
Acevedo, Raúl Maximiliano; Avico, Edgardo Hernán; Ruiz, Oscar Adolfo; Sansberro, Pedro Alfonso (Springer, 2018)Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, ... -
Gene expression changes in response to drought stress in Ilex paraguariensis leaves
Acevedo, Raúl Maximiliano; Ruiz, Oscar Adolfo; Sansberro, Pedro Alfonso (Southerm Cross, 2016-10)Ilex paraguariensis St. Hil. is recognized as a shade-tolerant tree, cultivated in north-eastern Argentina, south-eastern Brazil, and Paraguay. Its leaves and young shoots are industrialised and; hence, being used to prepare ... -
Isolation of high quality RNA from soil-grown Ilex paraguariensis roots suitable for next-generation sequencing and gene expression analyses
Avico, Edgardo Hernán; Acevedo, Raúl Maximiliano; Ruiz, Oscar Adolfo; Sansberro, Pedro Alfonso; Calzadilla, Pablo I. (Southerm Cross, 2017-07)Extraction of high quality RNA is a prerequisite for downstream application in functional genomics analyses. However, the extraction and purification of pure nucleic acids from root tissues is generally difficult due to ... -
Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
Acevedo, Raúl Maximiliano; Avico, Edgardo Hernán; González, Sergio; Acácio Rodrígues, Salvador; Rivarola, Máximo; Paniego, Norma; Nunes Nesi, Adriano; Ruiz, Oscar Adolfo; Sansberro, Pedro Alfonso (Springer Nature, 2019-05)Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, ...