In vitro antibacterial effect of argentine propolis extracts on streptococcus mutans and actinomyces viscosus
Fecha
2017Autor
Rosende, Roque Oscar
Lozina, Laura
Juárez, Rolando Pablo
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Aim: In the present study, the antibacterial activity of the Ethanol Extract of Propolis (EEP), collected
from various regions (Mendoza, Santiago del Estero, and Corrientes) in Argentina, against Streptococcus
mutans ATCC® 35668™ and Actinomyces viscosus ATCC® 15987™ (MicroBioLogics Inc., USA) was
investigated. Methods: Identification of geographic and botanical origin was based on a reconnaissance survey.
Phytochemical screening of propolis was carried out on ethanolic extracts using standard methods to identify
the constituents (aluminum chloride colorimetric method, Folin-Ciocalteu colorimetric method, thin layer
chromatography). The agar diffusion method (discs and wells) and serial dilution method (plates and tubes)
were used to evaluate the antibacterial activity of EEP. Results: EEP exerted various degrees of antibacterial
activity against S. mutans and A. viscosus, depending on the geographic area of collection. Phytochemical
screening showed that the bioactive compounds correspond to phenolic compounds and flavones. EEP from
Tunuyán (Mendoza), where the most abundant vegetation belongs to Populus sp., showed the highest content
of phenolic compounds (220.92±2.01 mg/g) and flavonoids (30.39±0.25 mg/g). This sample showed the most
profound antibacterial activity among the EEP tested. By the agar-well diffusion method, we found a high
susceptibility with an inhibitory halo of 11.25 ± 4.68 mm and 10.90 ± 4.21 mm against S. mutans and A.
viscosus, respectively. It also presented low Minimum Inhibitory Concentration and Minimum Bactericidal
Concentration values against S. mutans (MIC 0.05 mg/mL - MBC 0.46 mg/mL) followed by A. viscosus (MIC
0.11 mg/mL - MBC 0.93 mg/mL). Conclusions: The combined results from all methods indicated that S.
mutans is more susceptible to the effect of the Tunuyán EEP than A. viscosus.
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